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Photoelectric Colorimeter Operation Guide

This document discusses colorimetry and describes the components and basic operation of a photoelectric colorimeter. It is used to determine the concentration of colored compounds in a sample solution by measuring the amount of color. Key components include a light source, wavelength selector, filter, sample holder, cuvette, photodetector, and measuring device. It works based on Lambert's law where absorption is directly proportional to concentration. The document also briefly discusses constant pressure calorimetry and provides steps for operating a photoelectric colorimeter to determine the concentration of an unknown solution.

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Eng Cirro
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123 views2 pages

Photoelectric Colorimeter Operation Guide

This document discusses colorimetry and describes the components and basic operation of a photoelectric colorimeter. It is used to determine the concentration of colored compounds in a sample solution by measuring the amount of color. Key components include a light source, wavelength selector, filter, sample holder, cuvette, photodetector, and measuring device. It works based on Lambert's law where absorption is directly proportional to concentration. The document also briefly discusses constant pressure calorimetry and provides steps for operating a photoelectric colorimeter to determine the concentration of an unknown solution.

Uploaded by

Eng Cirro
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

machine :-

Col used to determine the concentration of colored compounds (analytes) in sample


solution .

color densitometers, which measure the density of primary colors, and color
photometers, which measure the color reflection and transmission.

-Lambert's law, according to which the absorption of light transmitted through the
medium is directly proportional to the medium concentration.

Light source
Monochromator/ wavelength selector
Filter
Solution/sample holder
Cuvette
Photosensitive detector system
Measuring device .

Constant Pressure calorimeter is used in determining the changes in enthalpy


occurring in solution. Under these conditions the change in enthalpy equals the
heat. And Measure H directly.

T is temperature change during reaction .

Steps for operating the photoelectric colorimeter:


1. Choose the glass filter recommended (see table below) in the procedure and insert in the
filter.
2. Fill two of the cuvette with blank solution to about three-fourth and place it in the cuvette
slot.

3. Switch on the instrument and allow it to warm up for 4 5 minutes.


4. Adjust to zero optical density.

5. Take the test solution i another cuvette and read the optical density.

6. Take the standard solution in varying concentration and note down the optical density as
S1, S2, S3, S4, S5 & so on.

Name : Hodan Mahamed Habane class : 2c


7. A graph is plotted taking concentration of standard solution versus the optical density.

8. From the graph the concentration of the test solution or the unknown solution can be
calculated.

[Link] distiller lab :-


Equipment used for water purification and distillation includes deionized (DI) water systems,
water distillers, reagent-grade water systems, and laboratory filter .

Name : Hodan Mahamed Habane class : 2c

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