0% found this document useful (0 votes)
85 views5 pages

DNA Fingerprinting Analysis and Results

This document contains tables and figures related to DNA fingerprinting analysis. It includes: 1) Tables showing DNA fingerprinting band data, standard curve calculations, and similarity matrices comparing different DNA samples. 2) Figures showing the standard curve graph and dendrograms comparing the relatedness of DNA samples using two different analysis methods. 3) The text describes the data and analysis presented in the tables and figures for determining genetic relationships between DNA samples.

Uploaded by

Addin Hayu P
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
0% found this document useful (0 votes)
85 views5 pages

DNA Fingerprinting Analysis and Results

This document contains tables and figures related to DNA fingerprinting analysis. It includes: 1) Tables showing DNA fingerprinting band data, standard curve calculations, and similarity matrices comparing different DNA samples. 2) Figures showing the standard curve graph and dendrograms comparing the relatedness of DNA samples using two different analysis methods. 3) The text describes the data and analysis presented in the tables and figures for determining genetic relationships between DNA samples.

Uploaded by

Addin Hayu P
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd

Gambar 3.

1 Visualisasi
Band DNA Fingerprinting.

f(x) = 0
R² = 0 Kurva Standar
2.5
f(x) = − 0.95 x + 2.38
2 R² = 1

1.5 BM standar
Log BM

Linear (BM standar)


1 BM yang dicari
Linear (BM yang dicari)
0.5

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
RF

Gambar 3.2 Kurva Standar.


Tabel 3.1 Penentuan Berat Molekul.
Rf (x) log BM (y) Antilog y (BM)
0,037879 2,3482886 222,9916681
0,068182 2,3194795 208,6793837
0,219697 2,1754341 149,7731938
0,25 2,146625 140,1602941
0,371212 2,0313886 107,4950922
0,560606 1,8513318 71,01201206
0,704545 1,7144886 51,8189533
0,825758 1,5992523 39,74223369
Tabel 3.2 Matriks n x t.
K/I A B C D E F G
1 - + + - - - -
2 + + - - - - -
3 - + - - + + +
4 + - + - + + +
5 - + - + + - -
6 - - - - - - -
7 - - - - + - -
8 - - - - + - -
9 + - - - + - -
10 - - + + - - -
11 - - + + - + +
12 - - - - + - -
13 - - + + - - -

Tabel 3.3 Matriks Indeks Similaritas Berdasarkan Simple Matching Coefficient.


A B C D E F G
A 1
B 0,615 1
C 0,538 0,462 1
D 0,462 0,538 0,769 1
E 0,538 0,462 0,231 0,308 1
F 0,692 0,615 0,692 0,615 0,538 1
G 0,692 0,615 0,692 0,615 0,538 1 1

Tabel 3.4 Matriks Node Berdasarkan Simple Matching Coefficient.


Objects in
Node Group 1 Group 2 Simil.
group
1 F G 1 2
2 C D 0,769 2
3 A Node 1 0,692 3
4 Node 3 B 0,615 4
5 Node 4 Node 2 0,577 6
6 Node 5 E 0,436 7
Tabel 3.5 Perbandingan Antara Node dengan Indeks Similaritas dan Koefisien
Korelasinya.
Node Anggota Sorted Unsorted Koefisien
1 FG 1 1 78,61022
2 CD 0,769 0,769
3 AF 0,692 0,692
AG 0,692 0,692
4 AB 0,615 0,615
FB 0,615 0,615
GB 0,615 0,615
5 AC 0,577 0,538
AD 0,577 0,462
BC 0,577 0,462
BD 0,577 0,538
FC 0,577 0,692
FD 0,577 0,615
GC 0,577 0,692
GD 0,577 0,615
6 AE 0,436 0,538
BE 0,436 0,462
CE 0,436 0,692
DE 0,436 0,308
FE 0,436 0,538
GE 0,436 0,538

Tabel 3.6 Matriks Indeks Similaritas Berdasarkan Jaccard's Coefficient.


A B C D E F G
A 1
B 0,167 1
C 0,143 0,125 1
D 0 0,143 0,5 1
E 0,25 0,222 0,091 0,1 1
F 0,2 0,167 0,333 0,167 0,25 1
G 0,2 0,167 0,333 0,167 0,25 1 1
Tabel 3.7 Matriks Node Berdasarkan Jaccard's Coefficient.
Objects in
Node Group 1 Group 2 Simil.
group
1 F G 1 2
2 C D 0,5 2
3 A E 0,25 2
4 Node 2 Node 1 0,25 4
5 Node 3 B 0,194 3
6 Node 5 Node 4 0,153 7

Tabel 3.8 Perbandingan Antara Node dengan Indeks Similaritas dan Koefisien
Korelasinya.
Node Anggota Sorted Unsorted Koefisien
1 FG 1 1 94,60976
2 CD 0,5 0,5
3 AE 0,25 0,25
4 CF 0,25 0,333
CG 0,25 0,333
DF 0,25 0,167
DG 0,25 0,167
5 AB 0,194 0,167
EB 0,194 0,222
6 AC 0,153 0,143
AD 0,153 0
AF 0,153 0,2
AG 0,153 0,2
EC 0,153 0,091
ED 0,153 0,1
EF 0,153 0,25
EG 0,153 0,25
UPGMA
E
D
C
B
G
F
A
0,4 0,5 0,6 0,7 0,8 0,9 1

Simple Matching Coefficient

Gambar 3.3 Dendogram Hasil UPGMA Dengan Metode Simple Matching Coefficient.

UPGMA
G
F
D
C
B
E
A
0,04 0,2 0,36 0,52 0,68 0,84 1

Jaccard's Coefficient

Gambar 3.4 Dendogram Hasil UPGMA Dengan Metode Jaccard’s Coefficient.

You might also like